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KMID : 0861020080230040081
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Korea Journal of Herbology
2008 Volume.23 No. 4 p.81 ~ p.89
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The Ethylacetate Extract of North Kangwhal(Ostericum koreanum) Attenuates the Inflammatory Responses in PMA/A23187-stimulated Mast Cells
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Seo Un-Kyo
Lee Ju-Il
Park Jun-Hong
Park Yong-Ki
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Abstract
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Objectives: In this study, the pharmacological effects of the ethylacetate extract of Ostericum koreanum(North Kangwhal; NK) on allergic inflammation were investigated in activated human mast cells.
Methods: North Kangwhal was extracted with 80% methanol for 24 h, and then fractionated with ethylacetate(NK-EtOAc extract). HMC-1 cells, an human mast line, were pre-incubated with different concentrations of NK-EtOAc extract for 30 min, and then stimulated with PMA(50 nM/ml) and A23187() at indicated times. The cell toxicity was determined by MTT assay. The concentrations of prostaglandin E2(PGE2) and cytokines(TNF-, IL-8) were measured by enzyme-linked immunosorbant assay.
Results: NK-EtOAc extract() significantly inhibited the productions of , TNF- and IL-8 in PMA/A23187-stimulated HMC-1 cells without cell toxicity(). NK-EtOAc extract also inhibited PMA/A23187-induced phosphorylation of ERK1/2 MAPK and the NF- p65 subunit translocation into the nuclear of HMC-1 cells.
Conclusions: This study suggests that NK-EtOAc extract may have an anti-inflammatory property through suppressing the production of inflammatory mediators in activated mast cells and its molecular mechanism underlies the blocking of NF- pathway.
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KEYWORD
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Ostericum koreanum, inflammation, mast cells, prostaglandin E2, cytokines, NF-
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